Expression, purification and crystallization of 2-­oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C

摘要

The gene encoding HpcG from the homoprotocatechuate (4-hydroxyphenylacetic acid) degradative pathway of E. coli C has been cloned and expressed and the protein has been purified. Crystals obtained from the purified recombinant enzyme, belonging to a tetragonal space group, diffracted to a resolution of 2.1 Å.